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Ultraconserved elements in the human genome: association and transmission analyses of highly constrained single-nucleotide polymorphisms.
Monday,2013-01-14 16:53 America/Los_Angeles — poolea2
| Title | Ultraconserved elements in the human genome: association and transmission analyses of highly constrained single-nucleotide polymorphisms. |
| Publication Type | Journal Article |
| Year of Publication | 2012 |
| Authors | Chiang, CWK, Liu, C-T, Lettre, G, Lange, LA, Jorgensen, NW, Keating, BJ, Vedantam, S, Nock, NL, Franceschini, N, Reiner, AP, Demerath, EW, Boerwinkle, E, Rotter, JI, Wilson, JG, North, KE, Papanicolaou, GJ, Cupples, AL, Murabito, JM, Hirschhorn, JN |
| Corporate/Institutional Authors | Genetic Investigation of ANthropometric Traits Consortium, |
| Journal | Genetics |
| Volume | 192 |
| Issue | 1 |
| Pagination | 253-66 |
| Date Published | 2012 Sep |
| ISSN | 1943-2631 |
| Keywords | Alleles, Animals, Body Height, Body Mass Index, Child, Conserved Sequence, Dogs, Evolution, Molecular, Female, Genetic Fitness, Genetic Variation, Genome, Human, Genotype, Humans, Inheritance Patterns, Male, Mice, Pedigree, Phenotype, Polymorphism, Single Nucleotide, Rats, Reproduction, Young Adult |
| Abstract | <p>Ultraconserved elements in the human genome likely harbor important biological functions as they are dosage sensitive and are able to direct tissue-specific expression. Because they are under purifying selection, variants in these elements may have a lower frequency in the population but a higher likelihood of association with complex traits. We tested a set of highly constrained SNPs (hcSNPs) distributed genome-wide among ultraconserved and nearly ultraconserved elements for association with seven traits related to reproductive (age at natural menopause, number of children, age at first child, and age at last child) and overall [longevity, body mass index (BMI), and height] fitness. Using up to 24,047 European-American samples from the National Heart, Lung, and Blood Institute Candidate Gene Association Resource (CARe), we observed an excess of associations with BMI and height. In an independent replication panel the most strongly associated SNPs showed an 8.4-fold enrichment of associations at the nominal level, including three variants in previously identified loci and one in a locus (DENND1A) previously shown to be associated with polycystic ovary syndrome. Finally, using 1430 family trios, we showed that the transmissions from heterozygous parents to offspring of the derived alleles of rare (frequency ≤ 0.5%) hcSNPs are not biased, particularly after adjusting for the rates of genotype missingness and error in the data. The lack of transmission bias ruled out an immediately and strongly deleterious effect due to the rare derived alleles, consistent with the observation that mice homozygous for the deletion of ultraconserved elements showed no overt phenotype. Our study also illustrated the importance of carefully modeling potential technical confounders when analyzing genotype data of rare variants.</p> |
| DOI | 10.1534/genetics.112.141945 |
| Alternate Journal | Genetics |
| PubMed ID | 22714408 |
| PubMed Central ID | PMC3430540 |
| Grant List | HHSN268201100012C / HL / NHLBI NIH HHS / United States HHSN268201100009I / HL / NHLBI NIH HHS / United States P30 ES010126 / ES / NIEHS NIH HHS / United States HHSN268201100010C / HL / NHLBI NIH HHS / United States HHSN268201100008C / HL / NHLBI NIH HHS / United States HHSN268201100005G / HL / NHLBI NIH HHS / United States HHSN268201100008I / HL / NHLBI NIH HHS / United States HHSN268201100005C / / PHS HHS / United States HHSN268201100007C / HL / NHLBI NIH HHS / United States HHSN268201100009C / / PHS HHS / United States HHSN268201100011I / HL / NHLBI NIH HHS / United States HHSN268201100011C / HL / NHLBI NIH HHS / United States UL1 TR000124 / TR / NCATS NIH HHS / United States HHSN268201100010C / / PHS HHS / United States HHSN268200960009C / / PHS HHS / United States HHSN268201100006C / HL / NHLBI NIH HHS / United States HHSN268201100008C / / PHS HHS / United States HHSN268201100012C / / PHS HHS / United States HHSN268201100005I / HL / NHLBI NIH HHS / United States N01HC65226 / HL / NHLBI NIH HHS / United States HHSN268201100007C / / PHS HHS / United States HHSN268201100009C / HL / NHLBI NIH HHS / United States HHSN268201100011C / / PHS HHS / United States HHSN268201100005C / HL / NHLBI NIH HHS / United States HHSN268201100007I / HL / NHLBI NIH HHS / United States HHSN268201100006C / / PHS HHS / United States N01-HC-65226 / HC / NHLBI NIH HHS / United States |